Description:

ABCG1(ATP-bindingcassettesub-familyGmember1),atransporterproteinprimarilyinvolvedinmacrophagelipidhomeostasis,localizestointracellularcompartmentsassociatedwithER/Golgimembranes,anditsexpressionishighestinmacrophage-richtissuesuchasspleen,lung,andthymusaswellasinbrain.ABCG1formheterodimerswithheterologousABCGpartnersandfunctionalheterodimerizationwithABCG4hasbeenofgreatinterestbecausebraincells,mainlyneuronsandastrocytes,depicthighexpressionofbothtransporters.ABCG1isinvolvedintheexportofavarietyoflipids(inconjunctionwithABCA1)includingcholesterolandphospholipidsfrommacrophagestoHDL.Macrophagelipidexportisthefirststepinthereversecholesteroltransportpathwaythatexportscholesterolfromperipheralcellstocirculatinglipoproteinsforeventualexcretionvialiver.Cholesterolexporttolipoproteinacceptorsiscompromisedinmacrophagefoamcells,oneoftheearlyhallmarksofatherosclerosis.Moreover,ABCG1playsroleinTcells/inflammation,brainlipidhomeostasisanditsexpressionincreasesinmacrophagesofTangierdiseasepatients.

Cat#+Size	6704-100
Size	100μl
AntibodyTarget	ABCG1
AlternateName	ABCG1;ABC8;WHT1;ATP-bindingcassettesub-familyGmember1;ATP-bindingcassettetransporter8;Whiteproteinhomolog
Host	Rabbit
AntibodyType	Polyclonal
Isotype	RabbitIgG
Immunogen	ThisABCG1antibodyisgeneratedfromrabbitsimmunizedwithaKLHconjugatedsyntheticpeptidebetween168-195aminoacidsfromtheN-terminalregionofhumanABCG1.
Accession#	P45844
GeneID	ABCG1
Appearance	Colorlessliquid
Formulation	100µlofantibodyinPBSwith0.09%(W/V)sodiumazide
Purification	ThisantibodyispreparedbySaturatedAmmoniumSulfate(SAS)precipitationfollowedbydialysisagainstPBS.
SpeciesReactivity	Human,mouse
Application	Westernblot,IHC,IF,FACS
PositiveControl	Westernblot:mousespleentissuelysate,IHC:humanlungcarcinoma,IF:293cells,FACS:HepG2cells
Application&Usage	Westernblot:~1:1000,FACS:~1:10–1:50,IHC:~1:50-1:100,IF:~1:10-1:50
Handling	Theantibodysolutionshouldbegentlymixedbeforeuse.
StorageConditions	-20°C
ShippingConditions	GelPack
USAGE	ForResearchUseOnly!NotForUseinHumans.