Description:

BioVision’sBSAispreparedbyutilizingtheCohnColdEthanolPrecipitationProcess.Thisprocessisoptimalforpreservingthenutrientfactorscriticaltotheapplicationsofcellculture,leptospiraandmicroBIOLOGicalgrowth.BSAmakesupapproximately60%ofallproteinsinanimalserum.Itiscommonlyusedincellcultureprotocols,particularlywhenproteinsupplementationisnecessaryandtheothercomponentsofserumareunwanted.ItaidsinstABIlizationofenzymesandotherproteinstopreventproteolysisanddenaturation,especiallywhentheproteinsarepresentinlowconcentrations.Incellcultureitsmainroleisasacarrierofsmallmolecules.Becauseofitsnegativecharge,BSAbindswater,salts,fattyacids,vitaminsandhormones,thencarriestheseboundcomponentsbetweentissuesandcells.ThebindingcapacityalsomakesBSAaneffectivescavengerforremovingtoxicsubstances,includingpyrogens,fromthemedium.

Highlights	SOURCE:TherawmaterialusedtomanufacturethislotwasderivedexclusivelyfromUSDA-regulatedabattoirslocatedintheUnitedStates.Theanimalsreceivedante-mortemandpost-morteminspectionunderalicensedveterinarian’ssupervisionandwerecertifiedatthetimeofslaughterasfreeofinfectiousorcontagiousdiseasesandinjuriousparasites. PURITY:≥98%bySDS-PAGEgelMOL.WEIGHT:~66.43kDa ENDOTOXINLEVEL:>0.3EU/mgbyGelClot FORM:Lightpowder OTHERPARAMETERS: pH(1%solution):7.0bypHmeteranalysis CalciumContent:<2=""mg/dl=""by=""aesenazo=""iii=""> ChlorideContent:<25=""meq/l=""by=""ise=""> PotassiumContent:<0.2=""meq/l=""by=""ise=""> SodiumContent:<20=""meq/l=""by=""ise=""> FattyAcid:1.1mg/gmbyEnzymaticcolorimetricanalysis ProteaseContent:BDLbyEIA MoistureContent:0.8%byThermogravimetricanalysis MicrobialContent:Passedlowerlimit.Growthmonitoredonaplateat30-35ºCfor48hrs. Mycoplasmacontent:NegativebyE.I.A.Qualitative ViralAgents(9CFR113):NoneDetected. SOLUBILITY:50mg/mlinwater,with15minofmanualstirring. APPLICATIONS/ADVANTAGES: •Canbeusedinmostsystemswhichdonotrequireaproteasefreeorenzymefreereagent. •Idealformakingdiluents,controlsandcalibrators/standards. •Applicableasaproteinbaseindiagnosticreagentsorwherenoproteolyticactivityisessential. •Reconstituteseasilyintoanopticallyclearandeasilyfilteredsolution.
StorageConditions	4°C
USAGE	ForResearchUseOnly!NotForUseinHumans.